Lymphocyte Separation via Density Gradient Centrifugation: A Reliable Technique for Researchers

Peripheral blood mononuclear cells (PBMCs), encompassing lymphocytes and monocytes, play a pivotal role in immunological investigations. Density gradient centrifugation (DGC) is a well-established technique for isolating PBMCs from whole blood, owing to the distinct buoyant densities of various blood cell populations. This article delves into the principles and practical considerations of DGC for researchers seeking a reliable method for lymphocyte enrichment.